Actividad antioxidante de extractos de hojas de Bocconia frutescens L. (Papaveraceae)

Oscar Eduardo Rodríguez Aguirre, William Alejandro Andrade Barreiro, Fabio Eduardo Diaz Lopez

Resumen


Bocconia frutescens es un árbol pequeño, posee hojas profundamente lobuladas y con borde dentado, las flores están agrupadas en panículas terminales pendulares, las semillas son de color negro brillante con una cubierta carnosa de color anaranjada. Es nativa de América, es conocida con los nombres vulgares de: Sarno, Palo Amarillo o Trompeto. Objetivos: Evaluar la actividad antioxidante de extractos y fracciones de hojas de Bocconia frutescens por los métodos DPPH• y ABTS•+. Métodos: las fracciones obtenidas por soxleth con solventes de diferente polaridad de hojas, fueron evaluadas a concentraciones de 25, 62.5, 125 y 250 mg/LMeOH para determinar la actividad antioxidante por los métodos DPPH• y ABTS•+. Resultados: Para los extractos por el método decoloración del radical DPPH• el Porcentaje de Captación se encontró entre 47.6 y 57.7 a 250 mg/LMeOH y para las fracciones entre 45.4 y 54.1 mg/LMeOH, a 250 mg/LMeOH. Por el método decoloración del radical ABTS•+. Para los extractos el porcentaje de Captación se encontro entre 89.7 y 99.7 a 250 mg/LMeOH y para las fracciones entre 68.5 y 99.6 mg/LMeOH. Conclusiones: las fracciones de acetato de etilo y metanol presentaron una alta actividad antioxidante al ser evaluadas por las técnicas ABTS•+ y DPPH• , la técnica ABTS•+ se presento mayor sensibilidad.

Abstract

Bocconia frutescens is a small tree, has deeply lobed and serrated edge leaves, flowers are grouped in pendulous panicles terminal, seeds are shiny black with a fleshy covering of orange color. It is native to America, is known under their common names: Sarno, Palo Amarillo or Trompeto. Objectives: Evaluate the antioxidant activity of extracts and fractions of leaves Bocconia frutescens by DPPH• and ABTS•+ methods. Methods: the fractions obtained by Soxhlet with solvents of different polarity of leaves, were evaluated at concentrations of 25, 62.5, 125 and 250 mg / LMeOH to determine the antioxidant activity by DPPH• and ABTS•+ methods. Results: Extracts for the bleaching method DPPH• radical the Percent Uptake was between 47.6 and 57.7 to 250 mg / LMeOH and fractions between 45.4 and 54.1 mg / LMeOH, to 250 mg / LMeOH. By the method of coloration radical ABTS•+. Extracts for the percentage uptake was found between 89.7 and 99.7 and fractions between 68.5 and 99.6 mg / LMeOH, to 250 mg / LMeOH. Conclusions: The fractions of ethyl acetate and methanol have a high antioxidant activity to be evaluated by the ABTS•+ and DPPH• techniques, the technique ABTS•+ present higher sensitivity.


Palabras clave


Antioxidante, Bocconia frutescens, ABTS•+, DPPH•, actividad antioxidante relativa (AAR).

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DOI: http://dx.doi.org/10.18270/rt.v14i2.1868

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